Always Hydrated
Natively Bioactive
Consistent Performance
NativFold™ FGF2 146
100μg
$252.00 USD
Sale price
$252.00 USD
Regular price
Product Specifications:
| Property | Specification |
|---|---|
| Source | E. coli |
| Amino Acid Sequence | Human FGF2 (Pro143-Ser288), Accession P09038 |
| N-terminal Sequence | Gly-Pro143 |
| Predicted Molecular Mass | 16.5kD |
| Activity | Measured in ERK activity and proliferation assays in 3T3 cells |
| Tag | Tag-free |
| Carrier | Carrier-free |
| Purity | >97% by stain-free SDS-PAGE |
| Endotoxin | <0.1 EU/µg |
| Formulation | 20 mM Tris-HCl, 150 mM NaCl, 10% trehalose pH 7.4 |
| Shipping | Dry ice |
| Storage | -80° C; avoid repeated freeze-thaws, aliquot after first thaw |
Biological Activity
NativFold™ FGF2 146 is functionally validated using ERK phosphorylation and cell proliferation assays in 3T3 cells, an FGF-responsive murine fibroblast line commonly used as the reference standard for FGF2 bioactivity testing. Biological activity is confirmed by dose-dependent induction of ERK1/2 phosphorylation and cell proliferation in a mitogenic-dependent manner.
Each lot is tested independently to ensure consistent bioactivity and lot-to-lot reproducibility.
FGF2 Stability Considerations in Cell Culture
Recombinant FGF2 has a half-life of approximately 10 hours at 37°C in cell culture media. To maintain sustained FGFR signaling during stem cell maintenance, supplemented cell culture media should be replaced every 24-48 hours.
To preserve Valor FGF2 146 bioactivity:
- Avoid repeated freeze-thaw cycles by aliquoting into single-use volumes immediately after the first thaw to maintain bioactivity and protein integrity.
- Store at -80°C; do not store at -20°C, to avoid temperature fluctuations that lead to protein degradation.
- Avoid exposing reagent to room temperature exposure during reagent preparation.
- Low-binding tubes and pipette tips should be used to avoid product loss.
- Adding 0.1% bovine serum albumin (BSA) as a carrier protein prevents surface adsorption.
- Do not vortex; gently flick or triturate with a pipette tip.
Recommended Working Concentrations
Working concentrations of FGF2 vary depending on cell type, culture conditions, and experimental objectives:
Recommended Working Concentrations
| Applications | Concentration Range |
|---|---|
| Induced pluripotent stem cells (iPSC) | 10-100ng/ml |
| Neural progenitor/stem cell culture | 10-50ng/ml |
| Mesenchymal stem cell culture | 1-10ng/ml |
| Chondrocyte expansion | 1-10ng/ml |
| Endothelial cell proliferation | 1-10ng/ml |
| Fibroblast proliferation assays | 1-20ng/ml |
| Smooth muscle cell culture | 1-10ng/ml |
Optimal concentrations should be determined for each experimental condition based on cell line responsiveness, media formulation, and experimental duration.
Applications
- Pluripotent stem cell (hPSC/iPSC) culture, maintenance, and expansion
- Mesenchymal stem cell (MSC) proliferation and expansion
- Neural progenitor and neural stem cell culture
- Endothelial cell growth and angiogenesis studies
- Chondrocyte expansion and cartilage tissue engineering
- Fibroblast proliferation assays
- Organoid and 3D cell culture systems
- Cell and gene therapy manufacturing
- Induced pluripotent stem cell (iPSC) reprogramming
- Receptor signaling and developmental biology research
- Serum-free and defined media formulations
- ELISA standard and immunoassay applications
For Research Use Only (RUO). Not intended for diagnostic or therapeutic applications.
Frequently Asked Questions:
NativFold FGF2-146 is not lyophilized, meaning it is never exposed to dehydration stress that damages protein structure and causes aggregation. Keeping the protein in a frozen liquid form preserves its hydration-dependent protein structure, leading to increased biological activity and reproducibility compared to typical freeze-dried products.
Each lot of NativFold FGF2-146 is validated by measuring ERK phosphorylation and cell proliferation assays in 3T3 cells. These assays confirm dose-dependent signaling and ensure consistent biological performance across lots.
NativFold FGF2 146 boasts over 97% monomeric purity, confirming that our proteins are in a bioactive, single-molecule form, which guarantees native bioactivity and reproducibility. Unlike legacy FGF2 products derived from insoluble aggregates treated with urea and harsh chemicals, which yield misfolded, aggregated proteins with reduced bioactivity, our monomeric FGF2 146 solution delivers dependable, consistent experimental outcomes.
In most cases, no protocol modifications are necessary. NativFold FGF2-146 can be used at the same concentration ranges as standard recombinant FGF2, making it a straightforward substitute.
Valor FGF2-146 is intended as a direct substitute for other commercial FGF2 products. It features the mature 146-amino acid isoform, which is responsible for receptor binding and signaling, as in other commercial products. This enables NativFold FGF2-146 to fit smoothly into current workflows while offering enhanced consistency and performance.
Absolutely. Valor's formulation is specifically formulated to reduce experimental variability. Because NativFold FGF2-146 remains stabilized in a hydrated state, it ensures reproducible signaling and consistent experimental outcomes.
The product should be stored at -80°C upon receipt to maintain stability. After the first thaw, it is recommended to aliquot the material into single-use volumes and store at -80°C to avoid repeated freeze-thaw cycles, which can reduce bioactivity. During handling, exposure to room temperature should be minimized, and low-binding tubes and pipette tips are recommended to prevent protein loss.
Our FGF2 is shipped on dry ice via overnight delivery to ensure the frozen liquid state protein's structural integrity. Orders are generally shipped Monday through Thursday to ensure safe delivery, unless alternative plans are in place for possible weekend delivery.
FGF2 has an approximate half-life of 10 hours at 37°C in cell culture media. To maintain consistent signaling, media supplemented with FGF2 is typically replaced every 24 to 48 hours, depending on the application and cell type.
We add 10% trehalose to NativFold FGF2 146 to prevent protein aggregation, stabilize the protein structure, cryoprotect, and improve recovery. While our formulation is optimized to maintain protein stability, for the best results, we recommend avoiding multiple freeze-thaw cycles and aliquoting into single-use volumes at -80°C after the first thaw.
When stored at -80°C under the recommended conditions, the product remains stable throughout its specified shelf life. Expiration details are included with each lot.
A certificate of analysis accompanies each lot, offering detailed data on purity, endotoxin levels, and biological activity to guarantee transparency and reproducibility.
NativFold FGF2-146 is meant solely for research use (RUO) and is not authorized for diagnostic or therapeutic purposes.
Bulk quantities and custom orders are offered to accommodate larger research initiatives, core facilities, and manufacturing processes. Please reach out to us at sales@valorsci.com to discuss your specific needs.
