Frequently Asked Questions:
NativFold FGF2-146 is not lyophilized, meaning it is never exposed to dehydration stress that damages protein structure and causes aggregation. Keeping the protein in a frozen liquid form preserves its hydration-dependent protein structure, leading to increased biological activity and reproducibility compared to typical freeze-dried products.
Each lot of NativFold FGF2-146 is validated by measuring ERK phosphorylation and cell proliferation assays in 3T3 cells. These assays confirm dose-dependent signaling and ensure consistent biological performance across lots.
NativFold FGF2 146 boasts over 97% monomeric purity, confirming that our proteins are in a bioactive, single-molecule form, which guarantees native bioactivity and reproducibility. Unlike legacy FGF2 products derived from insoluble aggregates treated with urea and harsh chemicals, which yield misfolded, aggregated proteins with reduced bioactivity, our monomeric FGF2 146 solution delivers dependable, consistent experimental outcomes.
In most cases, no protocol modifications are necessary. NativFold FGF2-146 can be used at the same concentration ranges as standard recombinant FGF2, making it a straightforward substitute.
Valor FGF2-146 is intended as a direct substitute for other commercial FGF2 products. It features the mature 146-amino acid isoform, which is responsible for receptor binding and signaling, as in other commercial products. This enables NativFold FGF2-146 to fit smoothly into current workflows while offering enhanced consistency and performance.
Absolutely. Valor's formulation is specifically formulated to reduce experimental variability. Because NativFold FGF2-146 remains stabilized in a hydrated state, it ensures reproducible signaling and consistent experimental outcomes.
The product should be stored at -80°C upon receipt to maintain stability. After the first thaw, it is recommended to aliquot the material into single-use volumes and store at -80°C to avoid repeated freeze-thaw cycles, which can reduce bioactivity. During handling, exposure to room temperature should be minimized, and low-binding tubes and pipette tips are recommended to prevent protein loss.
Our FGF2 is shipped on dry ice via overnight delivery to ensure the frozen liquid state protein's structural integrity. Orders are generally shipped Monday through Thursday to ensure safe delivery, unless alternative plans are in place for possible weekend delivery.
FGF2 has an approximate half-life of 10 hours at 37°C in cell culture media. To maintain consistent signaling, media supplemented with FGF2 is typically replaced every 24 to 48 hours, depending on the application and cell type.
We add 10% trehalose to NativFold FGF2 146 to prevent protein aggregation, stabilize the protein structure, cryoprotect, and improve recovery. While our formulation is optimized to maintain protein stability, for the best results, we recommend avoiding multiple freeze-thaw cycles and aliquoting into single-use volumes at -80°C after the first thaw.
When stored at -80°C under the recommended conditions, the product remains stable throughout its specified shelf life. Expiration details are included with each lot.
A certificate of analysis accompanies each lot, offering detailed data on purity, endotoxin levels, and biological activity to guarantee transparency and reproducibility.
NativFold FGF2-146 is meant solely for research use (RUO) and is not authorized for diagnostic or therapeutic purposes.
Bulk quantities and custom orders are offered to accommodate larger research initiatives, core facilities, and manufacturing processes. Please reach out to us at sales@valorsci.com to discuss your specific needs.
